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ecori avrii restriction enzymes  (InvivoGen)


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    Structured Review

    InvivoGen ecori avrii restriction enzymes
    Ecori Avrii Restriction Enzymes, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfuse2+clig+hl2/pFUSE2ss-CLIg-hL2/us12612449-745-39-35
    Average 94 stars, based on 31 article reviews
    ecori avrii restriction enzymes - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Identification of Novel Macropinocytosing Human Antibodies by Phage Display and High-Content Analysis
    Article Snippet: .. PCR-amplify heavy- and light-chain variable fragments separately from plasmid using appropriate primer sets (matching antibody sequences) and clone into either in-house expression vectors ( Smith, K., et al., 2009 ) or commercial heavy and light chain expression vectors such as pFUSE-CHIg-hG1 for human IgG1 heavy chain (InvivoGen) and pFUSE2-CLIg-hK (InvivoGen) for human kappa light chain or pFUSE2-CLIg-hL2 (InvivoGen) for human lambda 2 light chain. ..

    Article Title: Anti-PVRIG antibodies and methods of use
    Article Snippet: .. Protein expression constructs were derived by PCR-amplification of variable heavy, lambda and kappa domain genes, which were sub-cloned into pFUSE-CHIg-hG1 (human IgG1 heavy chain), pFUSE2-CLIg-hK (human kappa light chain) or pFUSE2-CLIg-hL2 (human lambda 2 light chain) vectors, respectively (all expression vectors were sourced from Invivogen). .. Expi293 cells (Life Technologies) were seeded at 6×105 cells/ml in Expi293 medium (Life Technologies) and incubated for 72 hrs at 37° C. in a humidified atmosphere of 8% CO2 with shaking at 125 rpm.

    Article Title: Anti-PVRIG antibodies and methods of use
    Article Snippet: .. Protein expression constructs were derived by PCR-amplification of variable heavy, lambda and kappa domain genes, which were sub-cloned into pFUSE-CHIg-hG1 (human IgG1 heavy chain), pFUSE2-CLIg-hK (human kappa light chain) or pFUSE2-CLIg-hL2 (human lambda 2 light chain) vectors, respectively (all expression vectors were sourced from Invivogen). .. Expi293 cells (Life Technologies) were seeded at 6×105 cells/ml in Expi293 medium (Life Technologies) and incubated for 72 hrs at 37° C. in a humidified atmosphere of 8% CO2 with shaking at 125 rpm.

    Article Title: PVRIG polypeptides and methods of treatment
    Article Snippet: .. Protein expression constructs were derived by PCR-amplification of variable heavy, lambda and kappa domain genes, which were sub-cloned into pFUSE-CHIg-hG1 (human IgG1 heavy chain), pFUSE2-CLIg-hK (human kappa light chain) or pFUSE2-CLIg-hL2 (human lambda 2 light chain) vectors, respectively (all expression vectors were sourced from Invivogen). .. Expi293 cells (Life Technologies) were seeded at 6×105 cells/ml in Expi293 medium (Life Technologies) and incubated for 72 hrs at 37° C. in a humidified atmosphere of 8% CO2 with shaking at 125 rpm.

    Plasmid Preparation:

    Article Title: Identification of Novel Macropinocytosing Human Antibodies by Phage Display and High-Content Analysis
    Article Snippet: .. PCR-amplify heavy- and light-chain variable fragments separately from plasmid using appropriate primer sets (matching antibody sequences) and clone into either in-house expression vectors ( Smith, K., et al., 2009 ) or commercial heavy and light chain expression vectors such as pFUSE-CHIg-hG1 for human IgG1 heavy chain (InvivoGen) and pFUSE2-CLIg-hK (InvivoGen) for human kappa light chain or pFUSE2-CLIg-hL2 (InvivoGen) for human lambda 2 light chain. ..

    Expressing:

    Article Title: Identification of Novel Macropinocytosing Human Antibodies by Phage Display and High-Content Analysis
    Article Snippet: .. PCR-amplify heavy- and light-chain variable fragments separately from plasmid using appropriate primer sets (matching antibody sequences) and clone into either in-house expression vectors ( Smith, K., et al., 2009 ) or commercial heavy and light chain expression vectors such as pFUSE-CHIg-hG1 for human IgG1 heavy chain (InvivoGen) and pFUSE2-CLIg-hK (InvivoGen) for human kappa light chain or pFUSE2-CLIg-hL2 (InvivoGen) for human lambda 2 light chain. ..

    Article Title: Anti-PVRIG antibodies and methods of use
    Article Snippet: .. Protein expression constructs were derived by PCR-amplification of variable heavy, lambda and kappa domain genes, which were sub-cloned into pFUSE-CHIg-hG1 (human IgG1 heavy chain), pFUSE2-CLIg-hK (human kappa light chain) or pFUSE2-CLIg-hL2 (human lambda 2 light chain) vectors, respectively (all expression vectors were sourced from Invivogen). .. Expi293 cells (Life Technologies) were seeded at 6×105 cells/ml in Expi293 medium (Life Technologies) and incubated for 72 hrs at 37° C. in a humidified atmosphere of 8% CO2 with shaking at 125 rpm.

    Article Title: Anti-PVRIG antibodies and methods of use
    Article Snippet: .. Protein expression constructs were derived by PCR-amplification of variable heavy, lambda and kappa domain genes, which were sub-cloned into pFUSE-CHIg-hG1 (human IgG1 heavy chain), pFUSE2-CLIg-hK (human kappa light chain) or pFUSE2-CLIg-hL2 (human lambda 2 light chain) vectors, respectively (all expression vectors were sourced from Invivogen). .. Expi293 cells (Life Technologies) were seeded at 6×105 cells/ml in Expi293 medium (Life Technologies) and incubated for 72 hrs at 37° C. in a humidified atmosphere of 8% CO2 with shaking at 125 rpm.

    Article Title: Elucidating the Molecular Mechanism of 3D1 Antibody Binding to a Swine Enteric Coronavirus Antigen
    Article Snippet: .. Briefly, codon-optimized genes for the respective formats were synthesized and cloned into mammalian expression vectors: the scFv construct into a pFUSE-based vector with a C-terminal 6xHis tag, and the Fab-Fc heavy and light chains into the pFUSE-hIgG1-Fc1 (InvivoGen, San Diego, CA, USA, #pfuse-hg1fc1) and pFUSE2-CLIg-hL2 (InvivoGen, San Diego, CA, USA, #pfuse2-hcll2). .. For protein expression, plasmids were transfected into HEK-293F cells using polyethylenimine (PEI, linear, MW 40,000, Yeasen, Shanghai, China, #40816ES03).

    Article Title: PVRIG polypeptides and methods of treatment
    Article Snippet: .. Protein expression constructs were derived by PCR-amplification of variable heavy, lambda and kappa domain genes, which were sub-cloned into pFUSE-CHIg-hG1 (human IgG1 heavy chain), pFUSE2-CLIg-hK (human kappa light chain) or pFUSE2-CLIg-hL2 (human lambda 2 light chain) vectors, respectively (all expression vectors were sourced from Invivogen). .. Expi293 cells (Life Technologies) were seeded at 6×105 cells/ml in Expi293 medium (Life Technologies) and incubated for 72 hrs at 37° C. in a humidified atmosphere of 8% CO2 with shaking at 125 rpm.

    Construct:

    Article Title: Anti-PVRIG antibodies and methods of use
    Article Snippet: .. Protein expression constructs were derived by PCR-amplification of variable heavy, lambda and kappa domain genes, which were sub-cloned into pFUSE-CHIg-hG1 (human IgG1 heavy chain), pFUSE2-CLIg-hK (human kappa light chain) or pFUSE2-CLIg-hL2 (human lambda 2 light chain) vectors, respectively (all expression vectors were sourced from Invivogen). .. Expi293 cells (Life Technologies) were seeded at 6×105 cells/ml in Expi293 medium (Life Technologies) and incubated for 72 hrs at 37° C. in a humidified atmosphere of 8% CO2 with shaking at 125 rpm.

    Article Title: Anti-PVRIG antibodies and methods of use
    Article Snippet: .. Protein expression constructs were derived by PCR-amplification of variable heavy, lambda and kappa domain genes, which were sub-cloned into pFUSE-CHIg-hG1 (human IgG1 heavy chain), pFUSE2-CLIg-hK (human kappa light chain) or pFUSE2-CLIg-hL2 (human lambda 2 light chain) vectors, respectively (all expression vectors were sourced from Invivogen). .. Expi293 cells (Life Technologies) were seeded at 6×105 cells/ml in Expi293 medium (Life Technologies) and incubated for 72 hrs at 37° C. in a humidified atmosphere of 8% CO2 with shaking at 125 rpm.

    Article Title: Elucidating the Molecular Mechanism of 3D1 Antibody Binding to a Swine Enteric Coronavirus Antigen
    Article Snippet: .. Briefly, codon-optimized genes for the respective formats were synthesized and cloned into mammalian expression vectors: the scFv construct into a pFUSE-based vector with a C-terminal 6xHis tag, and the Fab-Fc heavy and light chains into the pFUSE-hIgG1-Fc1 (InvivoGen, San Diego, CA, USA, #pfuse-hg1fc1) and pFUSE2-CLIg-hL2 (InvivoGen, San Diego, CA, USA, #pfuse2-hcll2). .. For protein expression, plasmids were transfected into HEK-293F cells using polyethylenimine (PEI, linear, MW 40,000, Yeasen, Shanghai, China, #40816ES03).

    Article Title: PVRIG polypeptides and methods of treatment
    Article Snippet: .. Protein expression constructs were derived by PCR-amplification of variable heavy, lambda and kappa domain genes, which were sub-cloned into pFUSE-CHIg-hG1 (human IgG1 heavy chain), pFUSE2-CLIg-hK (human kappa light chain) or pFUSE2-CLIg-hL2 (human lambda 2 light chain) vectors, respectively (all expression vectors were sourced from Invivogen). .. Expi293 cells (Life Technologies) were seeded at 6×105 cells/ml in Expi293 medium (Life Technologies) and incubated for 72 hrs at 37° C. in a humidified atmosphere of 8% CO2 with shaking at 125 rpm.

    Derivative Assay:

    Article Title: Anti-PVRIG antibodies and methods of use
    Article Snippet: .. Protein expression constructs were derived by PCR-amplification of variable heavy, lambda and kappa domain genes, which were sub-cloned into pFUSE-CHIg-hG1 (human IgG1 heavy chain), pFUSE2-CLIg-hK (human kappa light chain) or pFUSE2-CLIg-hL2 (human lambda 2 light chain) vectors, respectively (all expression vectors were sourced from Invivogen). .. Expi293 cells (Life Technologies) were seeded at 6×105 cells/ml in Expi293 medium (Life Technologies) and incubated for 72 hrs at 37° C. in a humidified atmosphere of 8% CO2 with shaking at 125 rpm.

    Article Title: Anti-PVRIG antibodies and methods of use
    Article Snippet: .. Protein expression constructs were derived by PCR-amplification of variable heavy, lambda and kappa domain genes, which were sub-cloned into pFUSE-CHIg-hG1 (human IgG1 heavy chain), pFUSE2-CLIg-hK (human kappa light chain) or pFUSE2-CLIg-hL2 (human lambda 2 light chain) vectors, respectively (all expression vectors were sourced from Invivogen). .. Expi293 cells (Life Technologies) were seeded at 6×105 cells/ml in Expi293 medium (Life Technologies) and incubated for 72 hrs at 37° C. in a humidified atmosphere of 8% CO2 with shaking at 125 rpm.

    Article Title: PVRIG polypeptides and methods of treatment
    Article Snippet: .. Protein expression constructs were derived by PCR-amplification of variable heavy, lambda and kappa domain genes, which were sub-cloned into pFUSE-CHIg-hG1 (human IgG1 heavy chain), pFUSE2-CLIg-hK (human kappa light chain) or pFUSE2-CLIg-hL2 (human lambda 2 light chain) vectors, respectively (all expression vectors were sourced from Invivogen). .. Expi293 cells (Life Technologies) were seeded at 6×105 cells/ml in Expi293 medium (Life Technologies) and incubated for 72 hrs at 37° C. in a humidified atmosphere of 8% CO2 with shaking at 125 rpm.

    Synthesized:

    Article Title: Elucidating the Molecular Mechanism of 3D1 Antibody Binding to a Swine Enteric Coronavirus Antigen
    Article Snippet: .. Briefly, codon-optimized genes for the respective formats were synthesized and cloned into mammalian expression vectors: the scFv construct into a pFUSE-based vector with a C-terminal 6xHis tag, and the Fab-Fc heavy and light chains into the pFUSE-hIgG1-Fc1 (InvivoGen, San Diego, CA, USA, #pfuse-hg1fc1) and pFUSE2-CLIg-hL2 (InvivoGen, San Diego, CA, USA, #pfuse2-hcll2). .. For protein expression, plasmids were transfected into HEK-293F cells using polyethylenimine (PEI, linear, MW 40,000, Yeasen, Shanghai, China, #40816ES03).

    Clone Assay:

    Article Title: Elucidating the Molecular Mechanism of 3D1 Antibody Binding to a Swine Enteric Coronavirus Antigen
    Article Snippet: .. Briefly, codon-optimized genes for the respective formats were synthesized and cloned into mammalian expression vectors: the scFv construct into a pFUSE-based vector with a C-terminal 6xHis tag, and the Fab-Fc heavy and light chains into the pFUSE-hIgG1-Fc1 (InvivoGen, San Diego, CA, USA, #pfuse-hg1fc1) and pFUSE2-CLIg-hL2 (InvivoGen, San Diego, CA, USA, #pfuse2-hcll2). .. For protein expression, plasmids were transfected into HEK-293F cells using polyethylenimine (PEI, linear, MW 40,000, Yeasen, Shanghai, China, #40816ES03).



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